Products

Using recombinant DNA and protein engineering technology, our company has developed a low-cost technique to produce, purify and refold recombinant human proteins.

Recombinant Human Bone Morphogenetic Protein-2 (rhBMP-2)

Package size:

10 ug per bottle.   US$100 per bottle.   US$90 per bottle for 2 bottles or more.

Bulk quotes on request.   Dealer enquiries are welcome.

Supplied:

Both solution and lyophilized powder from sterilized 50mM Acetic acid solution with or without BSA as a carrier protein approximately 50 ug per ug of BMP-2.

Production source:

1. E Coli

2. A cDNA sequence coding for human BMP-2 (284-396, ref. Science, 242: 1528-1534).

Molecular mass:

The mature recombinant human BMP-2 is a disulfide-linked homodimeric protein consisting of 113 amino acid residue monomer and has a calculated molecular mass of 12.8 kDa.

Purity:

Purified by inclusion body isolation, washing, iron exchange liquid chromatography and affinity chromatography with heparin-affinity chromatography. 

> 95% as determined by SDS-PAGE (silver stained).

Activity range:

Measured by its ability to induce alkaline phosphatase production during the process of conversion of C2C12 myoblast into osteoblast.

The ED₅₀ for this effect is approximately 50-500ng/ml.

Reconstitution:

Recommend to reconstitute the BMP-2 powder with sterile 50 mM acetic acid buffer, pH 4 or sterile PBS containing 0.1% human albumin or BSA to prepare a stock solution of no less than 10ug/ml.

Storage/Stability:

The lyophilized powder is stable for more than 2 years at -20 to -80 ºC or 6 months at 4 ºC. Upon reconstitution, the protein solution can be stored at 4 ºC for 2 months or aliquot stored at -80 ºC for one year.

Avoid repeated freeze-thaw cycles.

Disclaimer:

For laboratory use only. Not for use in or on humans. It is the responsibility of the user to comply with all local, state and federal regulations in the use of this product. We are not responsible for any patent infringements that might result from the use of or derivation of this product.